依据戊二醛与2，4-二硝基苯肼反应生成正戊醛二硝基苯肼，用高效液相色谱法，测定供试品中戊二醛含量。
照高效液相色谱法（通则0512）测定。
色谱条件 用十八烷基硅烷键合硅胶填充剂（C180，S-5μm，直径4.6mm，长250mm）；以70%乙腈溶液为流动相；流速为每分钟1.2ml；检测波长为360nm；记录时间为30分钟。
测定法 取戊二醛对照品适量，精密称定，加水溶解并定量稀释成每1ml中约含10μg的溶液；精密量取该溶液0.2ml、0.4ml、0.6ml、0.8ml、1.0ml，分别置试管中，各加水至1.0ml，精密加流动相1ml与2，4-二硝基苯肼溶液（称取2，4-二硝基苯肼2.4g，加30%高氯酸溶液，溶解成100ml） 0.1ml，立即于混合器上混匀，用0.45μm膜滤过。另取供试品适量，以每分钟3000转离心10分钟，精密量取上清液1ml，自“精密加流动相1ml”起，同法操作。分别精密量取对照品溶液与供试品溶液各10μl，注入液相色谱仪，记录色谱图。
以戊二醛对照品溶液的浓度对其相应的峰面积作直线回归，求得直线回归方程，计算出供试品溶液中戊二醛含量。
名称：液相色谱柱
型号：C18
规格：2.5um*4.6mm*150mm
应用：液相色谱法快速分析戊二醛消毒液的含量
Based on the reaction between glutaraldehyde and 2,4-dinitrophenylhydrazine to generate n-pentaldehyde dinitrophenylhydrazine, the content of glutaraldehyde in the test sample was determined by high-performance liquid chromatography.
Determine according to the high-performance liquid chromatography method (General Rule 0512).
Chromatographic conditions using octadecylsilane bonded silica gel filler (C180, S-5 μ m, diameter 4.6mm, length 250mm); Using 70% acetonitrile solution as the mobile phase; The flow rate is 1.2ml per minute; the detection wavelength is 360nm; and the recording time is 30 minutes.
Measurement method: Take an appropriate amount of glutaraldehyde reference substance, weigh it accurately, dissolve it in water and dilute it quantitatively to a solution containing about 10 μ g per 1ml; Accurately measure 0.2ml, 0.4ml, 0.6ml, 0.8ml, and 1.0ml of the solution and place them in test tubes. Add water to each tube until 1.0ml is reached. Accurately add 1ml of mobile phase and 0.1ml of 2,4-dinitrophenylhydrazine solution (weigh 2.4g of 2,4-dinitrophenylhydrazine, add 30% perchloric acid solution, dissolve into 100ml). Immediately mix well on a mixer and filter through a 0.45 μ m membrane. Take an appropriate amount of the test sample and centrifuge at 3000 revolutions per minute for 10 minutes. Accurately measure 1ml of the supernatant and proceed with the same procedure starting from "precision addition of 1ml of mobile phase". Accurately measure 10 μ l of the reference solution and 10 μ l of the test solution, inject them into the liquid chromatograph, and record the chromatogram.
Perform a linear regression on the peak area corresponding to the concentration of glutaraldehyde reference solution, obtain the linear regression equation, and calculate the glutaraldehyde content in the test solution.
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