浩瀚色谱（山东）应用技术开发有限公司通过玻璃球填充柱气相色谱法测定药典二氧化碳中碳氢化合物的含量，是一个成熟、专属性强的方法。其核心在于使用惰性的玻璃球填料色谱柱分离、FID检测器检测，并通过外标法定量，最终判断产品中碳氢化合物杂质是否低于0.0020%的限度。
所需材料与设备
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气相色谱仪系统：需配备氢火焰离子化检测器（FID） 和相应的气体进样阀或手动进样系统1。
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玻璃球色谱柱：这是核心组件。推荐使用0.8m长度 × 4mm内径，内填80目玻璃球的色谱柱（浩瀚色谱的HH-BLQ型号）。这种柱子是惰性的，对二氧化碳基质中的低碳数烃类（如甲烷）有良好的分离能力。
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高纯载气与检测器气体：通常使用高纯氮气或氦气作为载气。氢气和空气（或压缩空气）作为FID的燃烧气和助燃气。务必确保气体纯度，载气中的氧气含量最好不超过1 ppm，以免高温下损坏色谱柱固定相或影响基线。
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标准气体：甲烷标准气，其中甲烷在氮气中的浓度为0.0020%（体积分数），用于外标法定量36。
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待测二氧化碳样品：药典规定的二氧化碳样品
名称：玻璃球色谱柱
规格：0.8m*4mm，80目
zui高使用温度：300°C
应用：药典二氧化碳2020年版：碳氢化合物的测定
浩瀚色谱（山东）应用技术开发有限公司，研制生产的玻璃球色谱柱安装在岛津，安捷伦，赛默飞，瓦里安，布鲁克，PE等仪器上，柱效高，分离好，峰型对称，满足药典的指标要求。
适用于：
安捷伦490在线/便携，4890,5890,6890,7820,7890,8860,8890
岛津GC-14C，GC-2010，GC-2014，GC-2030
赛默飞1310,1300,1610,1600
瓦里安3800系列
布鲁克PE580,590,680,690
磐诺A90，福立GC9790，华爱，天瑞，天美，GC7900，川仪，北分瑞利，东西电子，鲁南瑞虹，捷岛/科晓
Haohan Chromatography (Shandong) Application Technology Development Co., Ltd. uses glass ball packed column gas chromatography to determine the content of hydrocarbons in pharmacopoeia carbon dioxide, which is a mature and highly specific method. The core lies in the use of inert glass ball packing chromatography columns for separation, FID detector detection, and quantification through external standard method to ultimately determine whether the hydrocarbon impurities in the product are below the limit of 0.0020%.
Required materials and equipment
Gas chromatograph system: A hydrogen flame ionization detector (FID) and corresponding gas injection valve or manual injection system 1 are required.
Glass sphere chromatography column: This is the core component. Recommend using a chromatography column with a length of 0.8m and an inner diameter of 4mm, filled with 80 mesh glass spheres (HH-BLQ model of Haohan Chromatography). This type of column is inert and has good separation ability for low-carbon hydrocarbons (such as methane) in the carbon dioxide matrix.
High purity carrier gas and detector gas: High purity nitrogen or helium gas is usually used as the carrier gas. Hydrogen and air (or compressed air) are used as combustion gases and auxiliary gases for FID. It is necessary to ensure the purity of the gas, and the oxygen content in the carrier gas should preferably not exceed 1 ppm to avoid damaging the chromatographic column stationary phase or affecting the baseline at high temperatures.
Standard gas: Methane standard gas, with a concentration of 0.0020% (volume fraction) of methane in nitrogen, used for external standard method quantification of 36.
Carbon dioxide sample to be tested: the carbon dioxide sample specified in the pharmacopoeia
Name: Glass Ball Chromatography Column
Specification: 0.8m * 4mm, 80 mesh
Maximum operating temperature: 300 ° C
Application: Pharmacopoeia Carbon Dioxide 2020 Edition: Determination of Hydrocarbons
Haohan Chromatography (Shandong) Application Technology Development Co., Ltd. has developed and produced glass sphere chromatography columns that are installed on instruments such as Shimadzu, Agilent, Thermo Fisher Scientific, Varian, Bruker, PE, etc. The columns have high column efficiency, good separation, symmetrical peak shapes, and meet the requirements of pharmacopoeia.
Suitable for:
Agilent 490 online/portable, 48905890689088608890
Shimadzu GC-14C, GC-2010, GC-2014, GC-2030
Thermo Fisher Scientific 1310130016101600
Varian 3800 Series
Brooke PE580590680690
Panno A90, Fuli GC9790, Huaai, Tianrui, Tianmei, GC7900， Chuanyi, Beifen Ruili, Dongxi Electronics, Lunan Ruihong, Jiedao/Kexiao
滕州市浩瀚色谱仪器技术服务有限公司
电话：0632-5667636
传真：0632-5667636
手机：15562228838,13963221227
地址：山东滕州市平行路（商务部和技术部）
邮箱：wangxiaoying9@126.com
QQ:1404939462
联系人：王经理
网址：www.haohansepu.com